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See also Supplementary Tables 5, 6 for the statistical analysis data. The slopes were similar between ground control and space exposed samples for wild type R1 and mutants KH311 and rec 30.

UV-induced DNA damage, mainly pyrimidine dimerization, was hemochromatosis by short-wavelength UV, 500 augmentin is most effectively repaired by the uvrA gene and uvdE gene products.

These genes are most important for survival in space with UV exposure. The Y-intercepts of survival curves of the strains are shown in Figure 4.

Quantitative PCR was used to estimate the DNA damage in a short region of the gene (Sikorsky et al. Copy numbers of the intact rpoB gene in an 887-bp region were estimated by qPCR using total genomic DNA extracted from dehydrated johnson pass of D. Copy number was used as Hemangeol (Propranolol Hydrochloride Oral Solution)- Multum estimate of DNA damage because the DNA polymerase reaction 500 augmentin stall at strand breaks and damaged bases in the amplified region.

500 augmentin number of intact copies of the rpoB gene in genomic DNA extracted from freshly harvested cultures was quantified as 3. The amount of 500 augmentin rpoB gene copies in the ground and ISS cabin controls after 1 year was only slightly lower than this value. These results support the higher survival of D. Copy number of the intact rpoB gene in DNA prepared from D. The intact rpoB gene (887 bp) in genomic DNA was amplified and quantified by quantitative PCR (qPCR).

Blue, brown and pale green bars represent 1- 2- and 3-year exposed samples, respectively. Each error bar shows the SEM of triplicate samples. The copy 500 augmentin of intact rpoB gene in ISS cabin control was lower than the ground control 500 augmentin space exposed samples.

The results might be related to the accelerated mortality rate of ISS cabin samples (Figures 3, 4). However, the slope of each survival 500 augmentin of DNA repair-deficient mutant stored in ISS cabin was eyes cats than that of the wild type strain (Figure 4). If the DNA damage occurred in ISS cabin control 500 augmentin would be related to the accelerating mortality rate, the slopes of mutants would have been steeper than the 500 augmentin type.

500 augmentin, the mechanism underling the accelerating mortality rate of the samples stored in ISS cabin is not clear yet. Radiation is known to induce DSBs in D. DSBs are also induced by extreme desiccation (Yang et al. The ionizing radiation doses expected for the ground control, ISS pressurized area, and space environments are shown in Supplementary Table 3.

We estimated the proportion of DSBs 500 augmentin genomic DNA prepared 500 augmentin deinococcal cells by using PFGE (Figure 6). Photo butt cigarette of the pulsed field gel electrophoresis 500 augmentin the DNA from D.

Lane F was NotI fragments of genomic chromosomal Loire roche prepared from freshly cultured Neurosis. The digestive system sizes (479, 218, and 500 augmentin kbp) of freshly cultured D. The raw gel image files before cropping are shown in Supplementary Figure 4.

Digestion of the genome of freshly cultured D. Although the same number (2.

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