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Membrane potential was measured as a ratio of red fluorescence (which depended on cell size and membrane potential) to green fluorescence (which depended on cell size alone). Each condition was measured in triplicate and each experiment was performed twice. Analyses were performed by the ANOVA test. A P value of less than 0. We thank John McKinney for the generous gift of the Erdman and icl mutant stains used herein and Carl Nathan, Sabine Ehrt, and Michael Malamy for helpful discussions.

This work was supported by National Institutes of Health AI081094, the Bill and Melinda Gates Foundation Grand Challenges Exploration program, and a Burroughs Wellcome Fund Career Award in the Biomedical Sciences (to K. Skip to main content Main menu Home ArticlesCurrent Special Feature Articles - Most Recent Special Features Colloquia Collected Articles PNAS Classics List of Issues PNAS Nexus Front MatterFront Matter Portal Journal Club NewsFor the Press This Week In PNAS PNAS in the News Podcasts AuthorsInformation for Authors Editorial and Journal Policies Submission Procedures Fees and Licenses Submit Submit AboutEditorial Board PNAS Staff FAQ Accessibility Statement Rights and Permissions Site Map Contact Journal Club SubscribeSubscription Rates Subscriptions FAQ Open Access Recommend PNAS to Your Librarian User menu Log in Log out My Cart Search Search for this keyword Advanced search Log in Log out My Cart Search for this keyword Advanced Search Home ArticlesCurrent Special Feature Articles - Most Recent Special Consciousness is Colloquia Collected Articles PNAS Classics List of Issues PNAS Nexus Front MatterFront Matter Portal Journal Club NewsFor the Press This Week In PNAS PNAS in the News Podcasts AuthorsInformation for Authors Editorial and Journal Policies Submission Procedures Fees and Licenses Submit Research Article Hyungjin Eoh and Kyu Y.

ResultsReplicative Quiescence of M. Metabolic Slowing and Remodeling of TCA Cycle Activity in M. We tested the specific role of the Hicon (Sodium Iodide I 131)- FDA shunt enzymes isocitrate lyase (icl1 and icl2, collectively referred to as ICL) as a mediator of the foregoing increase in succinate by comparing the metabolic profiles and in vitro survival of wild-type and ICL-deficient M.

Succinate-Mediated Maintenance of Membrane Potential in Hypoxic M. Nitrate-Dependent Modulation of TCA Cycle Activity in Hypoxic M. Metabolomics with Liquid Chromatography-Mass Spectrometry.

Metabolite identities were searched for using a mass tolerance of Isotopomer Data Analysis Using Isotope-Labeled Carbon Sources. The extent of isotopic labeling for metabolites was determined by dividing the summed peak height ion intensities of all labeled isotopologue species by the ion intensity of both labeled and unlabeled species, expressed in percentage.

Extraction of RNA industrial organizational psychologists Quantitative Real-Time PCR. AcknowledgmentsWe thank John McKinney for the generous gift of the Erdman and icl mutant stains used herein and Carl Nathan, Sabine Ehrt, and Michael Malamy for helpful discussions.

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OpenUrlCrossRefPubMedVoskuil MI, Visconti KC, Schoolnik GK (2004) Mycobacterium tuberculosis gene expression during adaptation Hicon (Sodium Iodide I 131)- FDA stationary phase and low-oxygen dormancy. OpenUrlCrossRefPubMedRustad TR, Harrell MI, Liao R, Sherman DR (2008) The enduring hypoxic response of Mycobacterium tuberculosis. Watanabe S, et al. OpenUrlCrossRefPubMedMarrero J, Rhee KY, Schnappinger Bacteriostatic Saline (Bacteriostatic NaCl)- FDA, Pethe K, Ehrt S (2010) Gluconeogenic carbon flow of tricarboxylic acid cycle intermediates is critical for Mycobacterium tuberculosis to establish and maintain infection.

OpenUrlCrossRefPubMedSchnappinger D, et al. OpenUrlCrossRefPubMedAly S, et al. OpenUrlCrossRefPubMedHeng Y, et al. OpenUrlCrossRefPubMedVia LE, et al. OpenUrlCrossRefPubMedEhrt S, Schnappinger Hicon (Sodium Iodide I 131)- FDA (2009) Mycobacterial survival strategies in the phagosome: Defence against host stresses. OpenUrlCrossRefPubMedSherman DR, et al.

OpenUrlCrossRefPubMedWayne LG, Lin KY (1982) Glyoxylate metabolism and adaptation of Mycobacterium tuberculosis to survival Hicon (Sodium Iodide I 131)- FDA anaerobic conditions. Energetics and mechanism of exchange, uptake and efflux. OpenUrlPubMedde Carvalho LP, Darby CM, Rhee KY, Nathan C (2011) Nitazoxanide disrupts membrane potential and intrabacterial pH homeostasis of Mycobacterium tuberculosis. OpenUrlCrossRefPubMedAlston TA, Mela L, Bright HJ (1977) 3-Nitropropionate, the toxic substance of Indigofera, is a Hicon (Sodium Iodide I 131)- FDA inactivator of succinate dehydrogenase.

OpenUrlCrossRefPubMedSohaskey CD, Wayne LG Hicon (Sodium Iodide I 131)- FDA Role of narK2X and narGHJI in hypoxic upregulation of nitrate reduction by Mycobacterium tuberculosis. OpenUrlCrossRefPubMedVander Heiden MG, Cantley LC, Thompson CB (2009) Understanding the Warburg effect: The metabolic requirements of cell proliferation.

Dimethyl succinate is used as a flavouring agent. It also has wide range of industrial applications such Voclosporin Capsules (Lupkynis)- Multum Functional how to care for dental veneers (open systems), Intermediates, Paint additives and Coating additives, Pigments Solvents, Viscosity adjustors.

HALS are used to inhibit degradation of polymers. DMS is also used as a pigment intermediate Dimethyl Succinyl Succinate (DMSS) CAS: 6289-46-9 for violet 19 and red 122. DMS is also used in Pharma applications. It has been used to form one step furan resins in subterranean wellbores, conductive pastes and even paper coatings and photographics.

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